Raising awareness, fostering education, and sharing knowledge across sectors, actors, and disciplines are fundamental to translating the One Health concept into practice. To support this goal, a One Health workshop was held during the 31st DACh-EPI Conference (Veterinary Epidemiology Conference for German-speaking countries) in 2024 in Salzburg, Austria. Designed with a practical format, the workshop engaged 20 participants from Austria, Germany and Switzerland through structured activities, including an interactive icebreaker, knowledge-sharing sessions with group discussions, a scenario-based challenge, and a focus group discussion. The main topics included the history and policy framework of One Health, strategies for overcoming implementation challenges, engaging policymakers, and examining real-world case studies. The participants collaboratively identified stakeholders, discussed best practices, and shared lessons learned from their own experience. The workshop outcomes, summarized in this report, highlight useful insights, shared challenges, and strategies for advancing the One Health approach. Additionally, a toolkit—including case studies, multimedia, and policy links—was provided to support ongoing application and learning. This workshop model offers a framework for engaging stakeholders and fostering cross-sectoral collaboration in implementing One Health policies.