Batrachochytrium salamandrivorans

Bsal

Changed on: 25.10.2021

Animal disease categories: D E

Profile

Amphibians are the most threatened class of vertebrates worldwide. In addition to biotope changes, fungal infections in particular lead to population collapses. The "salamander eater" or "salamander plague", a fungus (Batrachochytrium salamandrivorans), infects the skin of amphibians and leads to death, especially in caudates such as salamanders and newts.

Occurrence and hosts

Worldwide, the fungus was introduced from Asia to Europe via the trade in caudates. Endangered hosts are amphibians, especially caudates; frogs do not show symptoms but may be carriers of the fungal infection. In several European countries (Germany, Belgium, Spain, the Netherlands, Great Britain) there have already been mass mortalities of salamanders in the field and subsequently the extinction of entire populations in the infested regions.

Mode of transmision

In captivity, the fungus is transmitted to healthy animals through infected animals, furnishings and animal by-products from fungus-infected terrariums. In the field, the fungus can be introduced into a biotope via migrating caudates and frogs, via birds, via animals visiting the biotope and via humans.

Incubation period

7 days

Symptomatology

Highly contagious infection, which usually leads to death in caudate amphibians after more or less pronounced clinical symptoms (skin lesions, skin ulcers). 12-18 days after infection or 7 days after first symptoms, death occurs in caudate amphibians.

Therapy and hygiene measures

Tail amphibians in captivity can be treated with special fungicides. Treatments have been shown to be effective when kept at elevated temperatures.

Prevention

The EU is considering a trade and import ban on caudates from Asia. Tail amphibians purchased or bought in the trade must be carefully examined for fungal infections. They should be kept separate from existing captive animals by means of quarantine for the time being. Animal by-products associated with the keeping of caudates must be subjected to a heat treatment (10 days at 25 °C). Furnishings from terrariums that have come into contact with infected animals should be thoroughly cleaned and disinfected before reuse.

Situation in Austria

2016-2017: active monitoring; currently no infestation regions known in Austria

Professional information

Batrachochytrium salamandrivorans (Bsal), is a pathogenic fungus (genus: Batrachochytrium, order: Rhizophydiales, class: Chytridiomycetes) that mainly affects caudate amphibians - salamanders and newts. In regions where the fungus B. salamandrivorans has already been detected, these amphibians are considered endangered species due to mass mortality following fungal infections. Bsal has been detected in animals in the wild and in captivity in Germany (region: Eifel, Ruhr area - Essen area, Bavaria; 2018), Belgium (2013), Spain, the Netherlands (2008, 2013) and the UK.

B. salamandrivorans originates from Asia (Japan, China and Southeast Asia) and is not dangerous for the local amphibian fauna. In the course of evolution, the amphibians of Asia were able to adapt to the fungus. They show only a slight symptomatology. For the European caudate species, however, the fungus is new; the animals become infected, fall ill and die. The fungus is found in Europe in the wild and frequently in terrariums. Presumably, the fungus was introduced into Europe via the worldwide trade in exotic caudates and toads(Bombina microdeladigitora, Hubel's fire bellied toad). Exact details are not known, but trade remains the number one source of danger.

B. salamandrivorans is closely related to B. dendrobatidis (chytrid fungus, Bd). Unlike the chytrid fungus, the fungus forms a thallus with germ tubes. It occurs clustered in the form of colony-forming thalli. When mature, the thallus consists of a sporangium with germ tubes and rhizoids. The zoospores in the sporangium are motile and serve to spread the fungus. In addition, there are permanent spores that are resistant to environmental influences and can survive unfavourable environmental conditions in the soil or water over a longer period of time. The optimal ambient temperature for Bsal is 10-15 °C, after 5 days at 25 °C the fungus dies.

B. salamandrivorans poses a threat to the biodiversity of the European amphibian population. There is no known antidote against the spread of the fungus; infected populations must be considered lost. The EU has commissioned experts to set up a European warning system for the spread of the pathogen. Some European countries carry out nationwide monitoring (e.g. the Netherlands and the Czech Republic). In Austria, active monitoring was carried out from 2016-2017. The EU recommends the establishment of passive monitoring teams working according to a harmonized protocol. In some European countries with already established infestation regions, even caudates are removed from the population and transferred to a conservation breeding program.

Symptoms

The following caudate species have been shown to be affected by Bsal infection (see also EFSA 2017 Appendix E): the fire salamander(Salamandra salamandra), the North American fire salamander(S. algira), the Corsican salamander(S. corsica), the Asia Minor salamander (S. infraimmaculata) the Alpine (mountain) newt (Ichthyosaura alpestris), the pond newt(Lissotriton (syn. Triturus) vulgaris) and the filamentous newt(L. helveticus). The infection is highly contagious and almost always fatal to tailed newts. The disease may show a rapid course or, as in the case of the Alpine newt, a slow course. An infection in a biotope, in combination with other unfavourable factors, usually leads to the total loss of the population. A pathological infection of frogs (frogs and toads) is not known so far. However, frogs (e.g. the midwife toad Alytes obstetricans) can be intermediate hosts. They may carry the fungus on their skin and thus presumably act as reservoirs and vectors.

Clinical signs in caudate toads: Only adults are affected because the cutaneous fungus attacks the keratinous components of the skin, which are still absent during the larval stage. Infections with Bsal in fire salamanders are usually recognized by circular, black-rimmed, superficial skin lesions as well as deep skin ulcers. The skin ulcers are often colonized by bacteria. The severity of the skin lesions varies depending on the amphibian species. In newts, a more or less long symptom-free course of the disease is possible. In caudate amphibians, death occurs 12-18 days after injection or 7 days after the first symptoms appear, after a short phase of anorexia (loss of appetite), ataxia (disturbances in movement coordination), lethargy and apathy (apathy).

Literature

Allain St.J.R., Duffus A. Emerging infectious disease threats to European herpetofauna. Herpetological Journal 2019;29:189-206. doi.org/10.33256/hj29.4.189206.

Dalbeck L, et al. Salamander plague and its causative agent Batrachochytrium salamandrivorans (Bsal): current status in Germany. Journal of field herpetology 2018;25:1-22.

EFSA (European Food Safety Authority), Balaz V, et al. Scientific report: scientific and technical assistance concerning the survival, establishment and spread of Batrachochytrium salamandrivorans (Bsal) in the EU. EFSA Journal 2017;15(2):4739, 73 pp. doi:10.2903/j.efsa.2017.4739

EFSA AHAW Panel (EFSA Panel on Animal Health and Welfare), More S, et al. Scientific Opinion on the risk of survival, establishment and spread of Batracho-chytrium salamandrivorans (Bsal) in the EU. EFSA Journal 2018;16(4):5259, 78 pp. doi.org/10.2903/j.efsa.2018.5259

Mark Blooi et al: Treatment of urodelans based on temperature dependent infection dynamics of Batrachochytrium salamandrivorans. In: Scientific Reports 2015;5:Article 8037, doi:10.1038/srep08037.

Diagnostic

Skin lesions are not always visible in the early stages of infection, but usually only shortly before death. Molecular biological analyses (Duplex-qPCR, EFSA 2018) must therefore often be carried out in suspected cases. Preferred sample material in live animals are skin swabs. Mixed infections of Bsal and Bd occur.

The ELISA technique or cultivation of the fungus often leads to inaccurate results or false negatives and is therefore not recommended as a diagnostic tool (see EFSA 2017).In nature, Bsal infections are usually only noticed after deaths. In case of death, histological examination of the skin lesions can still be performed in addition to molecular biological analysis. While infestation with the chytrid fungus only results in hyperplasia or hyperkeratosis, fungal infections with Bsal also result in deep ulcers distributed over the body. The keratinocytes at the edges of the lesions are necrotic and contain a thallus.

Contact, Forms

Notification of deaths to:Institut für veterinärmedizinische Untersuchungen Mödling Robert Koch-Gasse 172340 MödlingTelephone: +43 50 555-38112Fax: +43 50 555-38529E-Mail: vetmed.moedlingno@Spam@agesno.Spam.at

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