National Reference Centre for Tuberculosis

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Changed on: 08.04.2019

Any tuberculosis diagnosis is designed to find the pathogen culture, as this is the only way to recognise the resistance pattern and investigate the outbreak. This requires a Biosafety Level 3 (BSL 3) laboratory, given the potential danger of the mycobacterium tuberculosis complex. The samples must be incubated for up to eight weeks as a result of the pathogen’s slow growth rate. However, molecular-biological techniques, such as Polymerase Chain Reaction (PCR), can shorten diagnosis time and provide clues to the resistance behaviour of the bacteria.

Latent tuberculosis infections can be diagnosed using the Mendel-Mantoux skin test, which has been used for decades, or blood tests that have been developed more recently, so-called interferon gamma release assays (IGRA). Unlike the tuberculin skin test, the blood test is not influenced by BCG vaccinations.

QuantiFERON-TB-Gold-Plus (IGRA)

The blood test QuantiFERON-TB-Gold® we currently conduct will be replaced by the newer QuantiFERON-TB-Gold-Plus® test by March 2016. This test will require four test tubes (grey, green, yellow and purple) instead of the three test tubes (grey, red and purple) required today. Sample taking or pre-analytics will not be affected by this change.

Sample taking and pre-analytics

All four test tubes must be filled up to the black marking (1ml each) and then shaken gently 10 times. The tubes should be incubated at 37° C as soon as possible, but at least within 16 hours. The test tubes should be stored at room temperature before incubation and MUST NOT be cooled or refrigerated. For further information on sample taking, pre-analytics and interpretation go to: www.quantiferon.com/irm/content/PI/QFT/PLUS/2PK-Elisa/UK.pdf

Contact

Mag. Dr. Alexander Indra
Phone: +43 50 555-37230
Währingerstraße 25a
1096 Wien
Mag. Dr. Alexander Indra
Phone: +43 50 555-37230
Währingerstraße 25a
1096 Wien

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