Propagation using Tissue Culture

Changed on: 10.11.2016

Cell and tissue culture uses the potential to regenerate entire plants from single cells or cell clusters (tissues) on specific culture media in a sterile, in-vitro environment.

Plant’s growth tissue is called meristem. Meristems consist of indeterminate cells which can evolve into any given plant cell. This is used in tissue culture: the meristems of the plant that should be propagated are removed from the tips of the shoots or the leaf axils and applied to specific nutrient media. They will grow into new, healthy plants.

Meristems are usually pathogen-free, thus, ideal for growing healthy parent plants. Plants can also undergo thermotherapy, in addition to meristem cuts, to destroy any existing pathogens, if necessary. Molecular biological tests can be used to examine whether there are still pathogens on the plant. This test is also required to produce pathogen-free seeds or propagation materials. 

Cell and tissue culture uses the potential to regenerate entire plants from single cells or cell clusters (tissues) on specific culture media in a sterile, in-vitro environment.

Plant’s growth tissue is called meristem. Meristems consist of indeterminate cells which can evolve into any given plant cell. This is used in tissue culture: the meristems of the plant that should be propagated are removed from the tips of the shoots or the leaf axils and applied to specific nutrient media. They will grow into new, healthy plants.

Meristems are usually pathogen-free, thus, ideal for growing healthy parent plants. Plants can also undergo thermotherapy, in addition to meristem cuts, to destroy any existing pathogens, if necessary. Molecular biological tests can be used to examine whether there are still pathogens on the plant. This test is also required to produce pathogen-free seeds or propagation materials. 

Service

AGES has many years of experience in the in-vitro propagation of agricultural crops (potatoes, elderberries, pome fruits, bananas), wood (willows, birches), ornamental plants (monstera, alocasia) and medicinal plants (great yellow gentian, cyclamens). As a result, AGES can offer the following services based on this experience:

  • Research and Development: development of methods for cell and tissue cultures of plants for which there are no methods up to now.
  • Creating small series of plant varieties that are difficult to cultivate under special conditions tailored to the species (from cultivation to the rooted plant acclimatised in the greenhouse)
  • Mass propagation of varieties that cannot or are difficult to grow from cuttings 
  • Meristem culture and thermotherapy for pathogen-free plant materials 
  • Development and optimisation of in-vitro culture protocols to culture protocols for the reinforcement and continued cultivation in a greenhouse (see Service Plant Health)
  • In-vitro, long-term storage of valuable plants away from climate and infection pressures caused by pathogens (conservation of genetic resources)

We have a modern greenhouse infrastructure (3,000 m² greenhouse area, 77 independent compartments, computer-operated climate, irrigation and fertilisation management, non-stop horticultural care), climate chambers, phytotrons, a container trial station and a lysimeter station, in addition to our in-vitro lab. 

Protection of species

Numerous crops and ornamental plants are subject to the Washington Convention, a treaty to protect endangered animal and plant species. This means that trade in such species is strictly limited or even prohibited. Thus, propagation using cell and tissue culture also serves as proof that plants grown using this method were not taken from the wild and were produced in line with international regulations on the protection of endangered species. Moreover, it is also a method to preserve endangered species that are difficult to propagate with cuttings or seeds.

Ornamental plants

Cell and tissue culture has many possible applications in cultivating ornamental plants: new varieties can be propagated more rapidly and varieties that are difficult to grow from cuttings can be grown more easily than using traditional methods. Phenotypical mutations – often intended in ornamental plants (e.g. different flower colours, leaf shapes or variegated leaves) – can be lost in conventional reproduction. This is also a field where in-vitro cultures can be used successfully.

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Alocasia
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Alocasia in-vitro
Alocasia In-vitro
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Alocasia: young plant potted
Alocasia: getopfte Jungpflanze
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Monstera
Monstera

Medicinal plants

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in-vitro mass propagation of great yellow gentian
In-vitro-Massenvermehrung von Gelbem Enzian

Another field of application is the propagation of medicinal plants for pharmaceutical purposes. The levels of active substances in medicinal plants may vary to large degrees, depending on climate and regional conditions, for example. Cell and tissue culture can assist in propagating plants with high levels of active substances under constant conditions.

Contact

DI Dr. Elisabeth Kopper
Phone: +43 50 555-34701
Spargelfeldstraße 191
1220 Wien



DI Dr. Elisabeth Kopper
Phone: +43 50 555-34701
Spargelfeldstraße 191
1220 Wien



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